Genetics

Test Cross

Contributors
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Ben Whitten

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What is a test cross?
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A test cross is a technique which is used by geneticists in which an individual whose genotype is unknown for a dominant phenotype (it could be homozygous dominant or heterozygous dominant) is crossed with an individual that is homozygous recessive at the locus in question.


The ratio of phenotypes in the offspring reveals the unknown genotype. 


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Illustrating a test cross: Drosophila melanogaster
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Drosophila melanogaster is the scientific name for the fruit fly, and is a good example of where test crosses are used as they have a large number of variants.


The long-winged condition is dominant (V) to the vestigial (non-useful) wing (v).


If a pure-breeding long-winged fly (VV) is mated with a vestigial-winged fly (vv), then the F1 generation (first filial) will all be heterozygous (Vv) and have long wings.


How can it be decided however whether a given F2 long-winged fly will be homozygous dominant (VV) or heterozygous (Vv)? This is determined by crossing the unknown genotype with a vestigial-winged fly.


As a vestigial-winged fly must be homozygous recessive (vv). If the long-winged fly whose genotype is unknown is VV, then crossing with the vv fly will produce all long-winged fly, however if the unknown fly is heterozygous (Vv) then there will be a mix of long and vestigial-winged flies in equal numbers (approximately).

Topic Menu
Introduction to Genetics
Monohybrid Cross
Test Cross
Multiple Alleles
Dihybrid Cross
Polygenic Inheritance
Dominance Patterns
Autosomal Inheritance
Sex-linked Inheritance
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